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. 2018 Feb 26;8:3580. doi: 10.1038/s41598-018-22044-y

Figure 2.

Figure 2

Active immunization with recombinant MntC induced specific IgG production. Female BALB/c mice (n = 4) were intramuscularly immunized with purified MntC. Seven days after the booster immunization, the mice were bled, and the sera were tested by ELISA for anti-recombinant protein antibodies. The titres were determined for (A) IgG, (B) IgG1, IgG2b, and IgG3. The results represent the mean and standard error of each group of mice. Significant differences were indicated by (*P < 0.05), (**P < 0.01) and (***P < 0.001).