Figure 3.
Opsonophagocytic activity mediated by immune sera from mice immunized with MntC. BALB/c mice (n = 5) were immunized with MntC or PBS, sera was separated. Source of peritoneal macrophages was MntC-vaccinated mice and unvaccinated mice respectively. 10 µL immune sera, 5 × 105 mouse peritoneal macrophages and 1 × 106 CFUs S. aureus Newman were incubated in round-bottomed six-well plates for 30 min before treating with agglutination lysis buffer and plating on TSA for CFUs. Then the percentage of phagocytosis was counted. PBS-vaccinated sera were used as negative control, and untreated sera were used as blank control. Significant differences were indicated by (*P < 0.05), (**P < 0.01) and (***P < 0.001).