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. 2018 Mar 1;16(3):e1002621. doi: 10.1371/journal.pbio.1002621

Fig 7. AID-induced lesions are more likely to undergo high-fidelity repair in PARP-1−/− cells.

Fig 7

(A) Mutation rate is limited in PARP-1−/− cells, even when AID expression is restored. Blue bars show AID expression levels (mean +/− SEM) before (dark blue) and after (light blue) transduction with ggAID cDNA as measured by Western blot and quantified by LICOR Odessey infrared imaging, normalized to β actin. Yellow bars show total mutation rates (mean +/− SEM) before (dark yellow) and after (light yellow) transduction with ggAID cDNA, measured as total mutations/bp/generation for the indicated cell lines. *** p < .0001, * p < .05, ns = not significant. (B) Increased AID expression restores mutation rate in hPARP to WT levels, but PARP-1−/− remain reduced. (C) Western blot showing PARP-1, AID, and β-actin levels at the beginning and the end of the culture period. (D) Mutation rate (mean +/− SEM when available) in PARP-1−/− cells with and without UGI expression, compared to hPARP cells. (E) Tables showing the distribution of mutations in PARP-1−/− and PARP-1−/−UGI cell lines. The changes are scored from the nucleotide indicated on the left to the nucleotide indicated on the top of the table.