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. 2018 Mar 1;13(3):e0192937. doi: 10.1371/journal.pone.0192937

Fig 2. Asymmetric illumination source enables phase-contrast.

Fig 2

(a) shows the transmission-function t(x) of the sinusoidial phase object and its spectrum which gets filtered in (b) by the WOTF of the brightfield microscope and in (c) by the DPC-system. One clearly sees, that an odd symmetric optical system is capable of transmitting phase information and images the phase-gradient.