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. 2018 Mar 1;13(3):e0193170. doi: 10.1371/journal.pone.0193170

Fig 5. Validation of protein levels of various gene products in our different mouse cohorts by immunoblotting.

Fig 5

(A) Example of western blots showing protein levels of Csad, Egfr, Fasn, and calreticulin in our mouse cohorts. (B) Signal intensity of ER marker calreticulin. (C) Ratio of Csad over calreticulin signal from the western blots. (Tukey post ANOVA test: †P < 0.05 compared to wild type mice). (D) Ratio of Egfr signal over calreticulin signal from the western blots. (ANOVA P-value = 0.07). (E) Ratio of Fasn signal over calreticulin signal from the western blots (ANOVA P-value = 0.33).