Table 3. Anticoagulant activities of chemically sulfated CSs and purified OSCS from contaminated heparin.
| IU/mg (95% Confidence Limits) | |||||
|---|---|---|---|---|---|
| sulfate level / disaccharide | Human APTT | Sheep APTT | Anti-Xa | Anti-IIa | HCII |
| CS 2.4 | 16 (15–17) | 41 (40–42) | <1 | <1 | 152 (134–173) |
| CS 3.0 | 52 (50–54) | 129 (126–132) | 1.4 (1.2–1.6) | 3.1 (2.9–3.3) | 753 (662–858) |
| CS 3.1 | 55 (54–56) | 141 (137–144) | 1.6 (1.4–1.8) | 4.2 (3.9–4.5) | 906 (796–1032) |
| CS 3.2 | 52 (50–54) | 138 (126–132) | 2.0 (1.8–2.2) | 3.7 (3.3–4.3) | 898 (788–1022) |
| CS 4.0 | 48 (47–49) | 137 (134–140) | 5.3 (4.8–5.9) | 4.5 (3.9–5.3) | 324 (259–404) |
| CS 4.0 (#2) | 50 (49–51) | 142 (139–145) | 5.9 (5.3–6.6) | 3.8 (3.5–4.0) | 264 (212–330) |
| Purified OSCS from Contaminated Heparin | 56 (54–57) | 181 (174–189) | 5.7 (5.2–6.3) | 4.9 (4.7–5.1) | 187 (166–210) |
| Chondroitin 4 sulfate | <1 | <1 | <1 | <1 | 1.9 (1.8–2.1) |
| Heparin sample | 209 (191–229) | 226 (219–233) | 214 (207–221) | 209 (196–223) | 241 (223–261) |
Values are IU/mg estimated against the 5th International Standard for unfractionated heparin (NIBSC, 97/578), with 95% confidence limits in brackets. All results were considered valid using multiple dilution models–slope ratio for plasma (human and sheep) assays and parallel line for purified protein (antithrombin, anti-IIa, anti-IIa, HCII anti-IIa) assays.