Fig 4. Altered myosin heavy chain or calcium handling protein expression are not affected by high-unsaturated fat diet.

Representative image of protein bands from (A) myosin heavy chain (MyHC), sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a), phospholamban (PLB), phosphorylated PLB at serine-16 (pPLB-Ser¹⁶), phosphorylated PLB at threonine-17 (pPLB-Thr¹⁷), and beta-actin (β-actin). (B) Quantification of the β-MyHC to α-MyHC isoforms. (C) Quantification of the SERCA2 normalized to β-actin. (D) Quantification of pPLB-Ser¹⁶ normalized to PLB. (E) Quantification of pPLB-Thr¹⁷ normalized to PLB. Data reported as fold change relative to Sham-Normo. * P <0.05 vs. respective sham; # <0.05 vs. AS-N, n = 6 each group.