Fig. 9.

6ʹ-GNTI–mediated inhibition of PGE₂-stimulated cAMP accumulation is blocked after disruption of DOR-KOR heteromers by DOR TM1-TAT peptides. Primary cultures of peripheral sensory neurons were treated with vehicle, (A) the DOR TM1-TAT peptide (1 µM), or (B) the negative control TAT-TM1 peptide (1 µM) for 30 minutes. Cells were incubated with PGE₂ (1 μM) and vehicle (Veh), 6ʹ-GNTI (100 nM), DPDPE (100 nM), or U50488 (100 nM) for an additional 15 minutes. Data are expressed as the percentage of PGE₂-stimulated cAMP levels and are mean ± S.E.M., n = 4. ****P < 0.0001 versus vehicle (no TM peptide), one-way ANOVA with Dunnett’s post test.