Fig. 4. Biliary release of uridine.

(A) Uridine concentrations in plasma and bile from 24 hour–fasted male C57BL/6 mice (n = 6). (B) Uridine concentration in bile from male and female C57BL/6 mice (n = 6 to 10 for each time point). (C) Biliary uridine levels in 24 hour–fasted male C57BL/6 mice fed with chow or HFD for 15 weeks (n = 6 per group). (D) Biliary uridine levels in 24 hour–fasted male WT and ob/ob mice fed with chow or HFD for 15 weeks (n = 6 per group). (E) Male C57BL/6 mice were administered with [5-³H]uridine by tail vein injection or oral gavage (gav). Plasma from tail tip and bile from gallbladder were harvested at indicated time points (n = 4 per time point). (F) Male C57BL/6 mice were administered with [³H]uridine by oral gavage. Plasma from tail tip and portal vein and bile from gallbladder were harvested 2 min after gavage (n = 6). Data were analyzed with two-tailed Student t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars denote SEM.