Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| Cell line (human) | HAP-1 parental | Horizon Discovery | RRID:CVCL_Y019 |
| Cell line (human) | HAP-1 p53 null | Horizon Discovery | HZGHC001068C007 |
| Cell line (human) | HCT116 parental | Invitrogen | HD PAR-007, RRID:CVCL_0291 |
| Cell line (human) | HCT116 R248W GOF | Invitrogen | HD 104–003 |
| Cell line (human) | H1299 p53 null | PMID: 27034505 | |
| Cell line (human) | H1299 WT | PMID: 27034505 | |
| Cell line (human) | H1299 S47 | PMID: 27034505 | |
| Cell line (human) | U2OS | ATCC | RRID:CVCL_0042 |
| Cell line (human) | SAOS-2 | PMID: 25024203 | |
| Cell line (human) | SAOS-2 R175H | PMID: 25024203 | |
| Cell line (human) | SAOS-2 R273H | PMID: 25024203 | |
| Cell line (mouse) | MEF p53 null | PMID:14702042 | |
| Cell line (mouse) | MEF WT | PMID:14702042 | |
| Cell line (mouse) | MEF R172H | PMID:15607981 | |
| Cell line (mouse) | MEF R172P | PMID:14702042 | |
| Cell line (mouse) | MEF p53 null | PMID: 27034505 | |
| Cell line (mouse) | MEF S47 | PMID: 27034505 | |
| Cell line (human) | MCF10a | Invitrogen | HD PAR-024, RRID:CVCL_0598 |
| Cell line (human) | MCF10a p53 null | Invitrogen | HD 101–005 |
| Antibody | IdU | BD Biosciences | 347580, RRID:AB_400326 |
| Antibody | CldU | Novus Biologicals | NB500-169, RRID:AB_10002608 |
| Antibody | Mouse Biotin | Sigma | B7653, RRID:AB_258625 |
| Antibody | Rabbit Biotin | Cell signaling | 5597S, RRID:AB_10828011 |
| Antibody | MLL3 | Abcam | ab32581, RRID:AB_881525 |
| Antibody | MRE11 | Abcam | ab214, RRID:AB_302859 |
| Antibody | RAD51 | Abcam | ab213, RRID:AB_302856 |
| Antibody | RAD52 | Santa Cruz | sc-365341, RRID:AB_10851346 |
| Antibody | GAPDH | Santa Cruz | sc-47724, RRID:AB_627678 |
| Antibody | Pol Theta | Abcam | ab80906, RRID:AB_1658691 |
| Antibody | PCNA | Santa Cruz | sc-56, RRID:AB_628110 |
| Antibody | ORC2 | Abcam | ab31930, RRID:AB_776911 |
| Antibody | p53 | Santa Cruz | sc-126, RRID:AB_628082 |
| Antibody | Ku70 | Cell Signaling | 4104S, RRID:AB_1904185 |
| Commercial assay or kit | Mouse plus PLA probe | Duolink Sigma | DUO92001-100RXN |
| Commercial assay or kit | Rabbit minus PLA probe | Duolink Sigma | DUO92005-100RXN |
| Commercial assay or kit | PLA detection reagent red | Duolink Sigma | DUO92008-100RXN |
| Chemical compound, drug |
EdU | Invitrogen | A10044 |
| Chemical compound, drug |
Hydroxyurea (HU) | Sigma | H8627 |
| Chemical compound, drug |
Thymidine | Sigma | T1895 |
| Chemical compound, drug |
IdU | Sigma | I7125 |
| Chemical compound, drug |
CldU | Sigma | C6891 |
| Chemical compound, drug |
Dynabeads MyOne streptavidin T1 |
Invittrogen | 65601 |
| Chemical compound, drug |
Alexa Fluor 488 Azide | Invitrogen | A10266 |
| Chemical compound, drug |
Cytochalasin B | Sigma | C2743 |
| Chemical compound, drug |
Formaldehyde solution | Sigma | 252549 |
| Chemical compound, drug |
Paraformaldehyde 32% solution, EM grade |
EMS | 15714 |
| Chemical compound, drug |
Goat Serum | Sigma | G9023 |
| Chemical compound, drug |
Biotin Azide | Invitrogen | B10184 |
| Chemical compound, drug |
DAPI | Life Technologies | 62248 |
| Chemical compound, drug |
Prolong Gold Antifade reagent |
Invitrogen | P36934 |
| Chemical compound, drug |
MTS | Promega | G3580 |
| Chemical compound, drug |
Mitomycin C | Sigma | M0503 |
| Software, algorithm | NIS elements | Nikon | RRID:SCR_014329 |
| Software, algorithm | Duolink Analysis tool | SigmaDuolink | RRID:SCR_015574 |
| Software, algorithm | GraphPad Prism 6 | GraphPad (La Jolla, California) |
RRID:SCR_002798 |
| Software, algorithm | Image J | RRID:SCR_003070 |