Figure 7. YTHDC2 localization in wild-type and mutant testes.

(A and B) YTHDC2 and SYCP3 immunofluorescence on testis sections from 2-month-old Ythdc2^em1/em1, Ythdc2^ketu/ketu, and their wild-type littermates. Arrowheads indicate SYCP3-positive spermatocytes. Scale bars represent 100 μm. (C) YTHDC2 and SYCP3 immunofluorescence on testis sections from an adult B6 male. Approximate seminiferous epithelial cycle stages (based on SYCP3 and DAPI staining patterns) are provided. S, Sertoli cell; Spg, spermatogonia; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; D, diplotene spermatocyte; RS, round spermatid; ES, elongating spermatid. Scale bar represents 15 μm.

Figure 7—figure supplement 1. YTHDC2 staining with an independent anti-YTHDC2 antibody in wild-type and mutant testes.

YTHDC2 and SYCP3 immunofluorescence on testis sections from 2-month-old Ythdc2^ketu/ketu and wild-type littermates. Figure labels are as in Figure 7. Scale bars represent 100 μm for panel A and 20 μm for panel B. In this figure, the anti-YTHDC2 antibody from Santa Cruz Biotechnology was used (Abby et al., 2016). In Figure 7, the anti-YTHDC2 antibody from Bethyl Laboratories was used (Soh et al., 2017).