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. 2018 Feb 27;9(3):326. doi: 10.1038/s41419-018-0347-x

Fig. 4. Inhibition of SMYD2 with its specific inhibitor, AZ505, delays tumor growth in vivo.

Fig. 4

a Tumor growth inhibition curve in female athymic nude mice bearing MDA-MB231 tumor xenografts treated intraperitoneally with either vehicle or AZ505 at doses described in Materials and Methods for fourteen consecutive days beginning on day 21 post implantation of 5 × 106 MDA-MB231 human breast cancer cells. Each point represents mean ± SD, n = 10. b and c Image (b) and graph (c) depicting the reduction in tumor size and tumor weight observed when MDA-MB231 implanted mice were treated with AZ505 compared to vehicle DMSO treated controls. Unpaired T-test was performed on tumor weight, p-value < 0.01, n = 10. d Tumor cell proliferation was decreased in xenografts from MDA-MB231 implanted mice treated with AZ505 compared to those treated with vehicle DMSO, as examined with Ki67 staining. The percentage of Ki67-positive nuclei was calculated in per mm2. p-value < 0.01. Scale bars, 100 μm. e AZ505 treatment induced tumor cell death in xenografts from MDA-MB231 implanted mice compared to those mice treated with vehicle DMSO, as examined by TUNEL assay. p < 0.01. Scale bars, 100 μm. f Tumor growth inhibition curve in female athymic nude mice bearing MDA-MB468 tumor xenografts treated intraperitoneally with either vehicle or AZ505 at doses described in Materials and Methods for fourteen consecutive days beginning on day 42 post implantation of 5 × 106 MDA-MB468 human breast cancer cells. Each point represents mean ± SD, n = 5. g and h Images (g) and graph (h) depicting the reduction in tumor size and tumor weight observed when MDA-MB468 implanted mice are treated with AZ505 compared to vehicle DMSO treated controls. Unpaired T-test was performed on tumor weight, p < 0.01, n = 5. i Tumor cell proliferation was decreased in xenografts from MDA-MB468 implanted mice treated with AZ505 compared to those treated with vehicle DMSO, as examined with Ki67 staining. The percentage of Ki67-positive nuclei was calculated in per mm2. p < 0.01. j AZ505 treatment induced tumor cell death in xenografts from MDA-MB468 implanted mice treated with AZ505 compared to those mice treated with vehicle DMSO, as detected by TUNEL assay. p < 0.01