Table 1. Validation of somatic and germline predictions.

Validation study	Call rate	Somatic variants predicted correctly	Germline variants predicted correctly
All variants in 30 lung & colon samples with matched-normal as gold standard (basic method)	100% (568/568)	67% (255/380)	87% (164/188)
All variants in 30 lung & colon samples with matched-normal as gold standard (SGZ)	85% (480/568)	95% (312/327)	99% (151/153)
All variants in 3 cell lines with varying proportions of tumor-normal admixture (basic method)	100% (215/216)	92% (83/90)	41% (51/125)
All variants in 3 cell lines with varying proportions of tumor-normal admixture (SGZ)	83% (184/222)	97% (60/62)	97% (118/122)
17 somatic hotspot mutations and 20 common germline variants in 20,182 clinical samples (basic method)	100% (12506/12506)	95% (7213/7560)	51% (2537/4946)
17 somatic hotspot mutations and 20 common germline variants in 20,182 clinical samples (SGZ)	84% (9829/11646)	96% (5325/5540)	97% (4172/4289)