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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Neurochem. 2017 Nov 20;144(1):58–67. doi: 10.1111/jnc.14244

Figure 4.

Figure 4

Non-octanoylated ghrelin (F203) binding in live hippocampal slice culture. a. Overnight incubation of live rat hippocampal slice in F302. b. Overnight incubation of live rat hippocampal slice in F203. c. Overnight incubation of GHSR1a KO live slice in F302. d. Overnight incubation of GHSR1a KO live slice in F203. e. Overnight incubation of fixed rat slice culture in F302. f. Overnight incubation of fixed rat slice culture in F203. g. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F302. h. Overnight incubation of live rat hippocampal slice in GOAT inhibitor with F203. i. Summary of results: F302 (n=15 rats), F203 (n=15 rats), F302 in GHSR1a KO (n=7 mice), F203 in GHSR1a KO (n=7 mice), F203 in fixed slices (n=7 rats), and F203 in GOAT inhibitor (n=7 rats). Inset in i depicts FITC signals in red ROIs. Calibrations: 300 μm in a (shared with b, c, and d). 400 μm in e (shared with f, g, and h). * p<0.001.