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. 2018 Jan 11;10(2):390–405. doi: 10.1016/j.stemcr.2017.12.012

Figure 7.

Figure 7

BMP4 Also Directs iPSCs toward a dI1s and dI3 Identity

(A–H) Day 36 EBs, derived from iPSCs treated with RA and RA + BMP4-D10, were either processed for RT-qPCR or labeled with antibodies against Tuj1 (red), LHX2 (green, A and B), ISL1 (green, E and F), and DAPI (blue).

(A–D) The BMP4-D10 condition resulted in a significant increase in LHX2 mRNA and number of LHX2+ Tuj1+ dI1s in both hESC-derived EBs (C, probability similarity with RA control p < 0.023; D, p < 0.0001) and iPSC-derived EBs (C, p < 0.0096; B′ and D, p < 0.0001) compared with RA control (arrow, A′ and D).

(E–H) The BMP4-D10 condition also resulted in a significant increase in ISL1 expression in hESCs-EBs (G, p < 0.016) and iPSC-EBs (G, p < 0.04) and the number of ISL1+ Tuj1+ dI3s (hESCs-EBs: H, p < 0.0005; iPSC-EBs: F′ and H, p < 0.0022), compared with RA control (E′, G, and H).

Three biological replicates were performed. Probability of similarity: ∗∗p < 0.005, ∗∗∗p < 0.0005. Three biological replicates were performed with 10–18 EBs quantified. The number of cells was normalized and then scaled according to a unit area (10,000 μm2). Scale bar, 100 μm.