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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Comp Neurol. 2017 Apr 18;525(10):2287–2309. doi: 10.1002/cne.24215

FIGURE 4.

FIGURE 4

Bar contains predominantly glutamatergic neurons, as shown by the presence of a GFP reporter for the type 2 vesicular glutamate transporter (Vglut2), and absence of a reporter for the vesicular GABA/glycine transporter (Vgat). Panels (a) and (b) compare the distributions of Vglut2 (glutamatergic) and Vgat (GABAergic/glycinergic) neurons, respectively, in the pontine tegmentum at levels containing the core of Bar. TH immunofluorescence (magenta) is labeled as a landmark. The core of Bar contains a prominent cluster of glutamatergic neurons, and is virtually devoid of GABAergic neurons. Conversely, a region of the pontine central gray (PCG) just medial and ventral to Bar contains many Vgat/GABAergic neurons and relatively few Vglut2/glutamatergic neurons. Scale bar in (b) is 200 μm and applies to both panels. Additional abbreviations: Cb = cerebellum; DR = dorsal raphe nucleus; DTN = dorsal tegmental nucleus; Me5 = mesencephalic trigeminal nucleus; Su5 = supratrigeminal nucleus