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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Neurosci Res. 2017 Sep 1:10.1002/jnr.24152. doi: 10.1002/jnr.24152

Figure 1.

Figure 1

Gaussia luciferase (GLuc) variants. A: Comparison of amino acid sequences of wild type GLuc (WT; position 48 – 185; GenBank number AAG54095.1) to ‘superluminescent’ (SL; Kim et al., 2011), ‘slow burn’ (SB; Welsh et al., 2009), and GLucM23 (M23; Lindberg et al., 2013). Amino acid changes are highlighted. B: Comparison of bioluminescence emission of GLuc variants. HEK293 cells were lipofected with pcDNA3.1 plasmids of the different GLuc variants fused to VChR1-EYFP. Fluorescence of the EYFP reporter was used to normalize luminance from the GLuc variants. CTZ was added to a final concentration as indicated. Luminance was measured in an IVIS system. The intensity of light emission is represented by pseudo-colors, with red areas representing the highest amount of light emission, and blue/violet areas representing the least. C: Comparison of radiance of GLuc variants at 50 μM concentration of CTZ. IVIS measurements of 4 individual samples for each variant were graphed. GLucM23 emits significantly more photons than either wild type GLuc (P = 0.002235) or superluminescent GLuc (P = 0.007356).