Skip to main content
. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Orthop Res. 2017 Sep 20;36(2):788–798. doi: 10.1002/jor.23715

Figure 1. Study Design.

Figure 1

Six coccygeal IVD motion segments from 12-week-old mice were harvested and used for a 14-day organ culture experiment assessing the effects of osmolarity on the NP phenotype. An initial 3-day stabilization step was implemented for all samples to reach a steady-state condition after dissection, ensuring equal initial conditions. The following 11-day osmotic application step involved two osmolarity conditions: hyperosmolarity (Hyper) and hypoosmolarity (Hypo); and three durations: Cyclic, Burst and Static. Day 0 (Control-Day0) and time-matched (Control-Day14) controls were used. Culturing conditions included daily media changes for similar nutrition levels per group.