Figure 3. Immunostaining for NC phenotypic markers persisted in all NC and sNPCs across loading groups.
The Hyper-Static samples, which had predominantly NCs are compared with Control-Day0, Control-Day14 and Hypo-Static groups which had predominantly sNPCs. Expression for known NC-phenotypic markers cytokeratin 8 (Ck8), cytokeratin 19 (Ck19), and sonic hedgehog (Shh) persisted in all NP cells. Images shown for groups exhibiting largest cell morphological changes to NP cells. Scale bar 100um and 20um.