Fig. 2.

Upregulation of Glo1, phospho-JNK and KLF4 occurs in endothelial cells lining human CCM lesions. Histological sections of human CCM surgical specimens deriving from KRIT1 loss-of-function mutation carriers were analyzed by immunohistochemistry (IHC). Glo1 (a,b), phospho-JNK (c,d) and KLF4 (e,f) IHC staining of a representative CCM surgical sample containing large CCM lesions lined by a thin endothelium (a,c,e), and perilesional normal vessels serving as internal negative controls (b,d,f). Original magnification: ×400. Notice that a significant positive IHC staining is evident for both Glo1, phospho-JNK and KLF4 in endothelial cells lining the lumen of CCM lesions (panels a,c,e, respectively, red boxes and arrows), as compared with endothelial cells lining the lumen of perilesional normal vessels (panels b,d,f, respectively, red boxes). Scale bars: a,c,e, 30 µm; b,d,f, 200 µm.