Fig. 4.

Diaph1 deletion downregulated EGR1 expression. (a) Diaph1 deletion decreased nuclear EGR1/histone 3 after LAD/reperfusion in mouse hearts (n = 4/group; *p < 0.05 vs. WT LAD) and (b) Diaph1 silencing decreased nuclear EGR/nucleoporin p62 in H9C2 cells after H/R (n = 5/group; *p < 0.05 vs. shScr H/R). (c) ChIP studies revealed an interaction between SRF protein and the Egr1 promoter region in WT but not Diaph1^–/– hearts following LAD/reperfusion (n = 3/group). Unpaired two-tailed t-tests were used; a p-value < 0.05 was considered significant.