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. 2017 Nov 3;26:100–111. doi: 10.1016/j.ebiom.2017.10.030

Fig. 2.

Fig. 2

Inhibitory effects of deguelin on the growth of ESCC cells. (a) The chemical structure of deguelin. (b) Cytotoxicity of deguelin was measured in normal esophageal epithelial cells by MTS assay. Het-1A cells were treated with various concentrations of deguelin for 24 or 48 h. Data are shown as means ± S.D. from triplicate experiments. (c) The viability of ESCC cells was measured by MTS assay in KYSE180 (left), KYSE150 (middle) and Eca109 (right) cancer cells. All ESCC cells were treated for 24, 48 and 72 h with various concentrations of deguelin as indicated. Data are shown as mean values ± S.D. from 3 independent experiments conducted with triplicate samples. Statistical significance was determined by the Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001 vs. DMSO-treated group). (d) A colony formation assay was performed as described in the “Materials and Methods” section. Data shown are the colony formation ability of KYSE180 (top), KYSE150 (middle) and Eca109 (bottom) cells treated with different concentrations of deguelin compared with the DMSO group. The average colony number was calculated from three separate experiments (*p < 0.05, **p < 0.01, ***p < 0.001 vs. DMSO-treated group).