Skip to main content
. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Mol Carcinog. 2018 Jan 11;57(4):512–521. doi: 10.1002/mc.22776

Figure 2. Nrf2 knockdown attenuated the inhibitory effects of CRA on the anchorage-independent growth of TRAMP-C1 cells.

Figure 2

Stable mock (scramble-sequence control, sh-Mock) and Nrf2 knockdown (sh-Nrf2) TRAMP-C1 cells were established using lentivirus mediated short hairpin RNAs and were selected with puromycin for 3 weeks. (A) Reduced mRNA expression of Nrf2 in knockdown cells was confirmed by qPCR. (B) Anchorage-independent growth of sh-Mock and sh-Nrf2 with or without the presence of CRA for 14 days. (C) Representative images of each group under a microscope. All the data are presented as the mean ± SEM. *P < 0.05 compared to the control.