Figure 3. CRA increased the mRNA expression levels of Nrf2 and Nrf2 target genes in TRAMP-C1 cells.
TRAMP-C1 cells were treated with CRA (2, 4 and 8 µM) for 5 days. After treatment, mRNA was isolated from the TRAMP-C1 cells, and the expression levels of target genes were determined using real-time qPCR assays with β-actin as an internal control. The graphical data are expressed as the mean ± SEM from three independent experiments. *P < 0.05 compared to the control.