Figure 5. Cell growth properties of MDA-MB-231 cells incubated with 5-HDST.
Bars/symbols represent means ± SEM from three separate determinations performed in triplicate (N=3). A. Cell number (percent of vehicle) measured after incubation (1–6 days) with 5-HDST. B. Anchorage-dependent colony formation following incubation with 5-HDST for 14 days. C. Anchorage-independent colony formation following incubation with 5-HDST for 14 days. D. Transwell assays to measure migratory ability of scrambled shRNA cell line incubated with 5-HDST. One-way ANOVA was conducted to compare relative invasive ability between groups (p<0.05). The scrambled shRNA cell line incubated with 100 μM 5-HDST had significantly (p<0.05) less invasive ability compared to scrambled shRNA cell line incubated with vehicle following one-way ANOVA and Bonferroni post hoc test (p<0.05).