(a) Representative conformations of a membrane with κ = 20 kBT and
as a function of ℒt. Panels (b) and (c) show the computed values of the tether length lt, and radius ℛt, respectively, as a function of ℒt. These quantities are computed as described in the methods section. The shaded regions mark the three regimes for initial tether formation (i.e., for lt/ℛt < 5) namely, regime 1: suppression of undulations, regime 2: formation of tethers, and regime 3: extrusion of tethers at a constant radius. The boxed numbers in the top panel denote the regimes to which the configurations correspond to.