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. 2018 Feb 21;285(1873):20172685. doi: 10.1098/rspb.2017.2685

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — CRISPR/Cas9 mosaic wing pattern phenotypes of apA knockouts. (a) Top: regions of the apA gene in B. anynana targeted using the CRISPR/Cas9 system. Bottom: sequences of the homeodomain and LIM domain regions of mutant individuals compared with the wild-type sequence in bold. Blue is the region targeted and the PAM sequence is in red. Deletions are indicated with ‘-’. (b) A subset of the CRISPR/Cas9 apA mutant phenotypes observed in B. anynana. The left column shows the wild-type (WT) dorsal and ventral surfaces for male forewings and hindwings. M9-12 (top): the dorsal forewing of a mutant male highlighting some of the ventral-like phenotypes and defects. The boxed regions are expanded to show the appearance of ventral-like white band and silver scales. M9-3: dorsal forewing surface of a mutant female resembling the ventral surface. M9-27: mutant with the ventral teardrop shape forewing androconial organ appearing on the dorsal surface (red arrow). WT dorsal forewing androconia is shown for comparison. M9-12 (bottom): a mutant dorsal hindwing with the appearance of all seven eyespots (red arrows), normally only seen on the ventral surface. The boxed regions are expanded to show the loss of silver scales associated with the dorsal hindwing androconia and improper development of hair-pencils. WT hair-pencil is shown for comparison. M9-2: mosaic phenotype (left) on the dorsal surface with ventral-like light coloured scales. Clones are indicated with a dashed white line. Corresponding region of the other wing of the same individual (right) shows no mosaicism. M235-11: a dorsal hindwing of a mutant with the width of the gold ring resembling that of ventral eyespots. (Online version in colour.)