Table 1.

Formulation composition and obtained responses of 15 different formulation obtained from Box–Behnken design. X₁ = amount of lipid, X₂ = amount of surfactant, X₃ = amount of drug, Y₁ = particle size, Y₂ = PDI, Y₃ = zeta potential, Y₄ = AE and Y₅ = DL.

	independent variables			dependent variables
formulation	X1 (mg)	X2 (mg)	X3 (mg)	Y1 (nm)	Y2	Y3 (mV)	Y4 (%)	Y5 (%)
F1	250	60	7	208.0	0.127	−28.04	67.8	1.88
F2	350	60	7	236.5	0.158	−32.31	61.9	1.26
F3	250	100	7	169.0	0.174	−23.17	67.0	1.85
F4	350	100	7	195.0	0.158	−23.62	62.4	1.57
F5	250	80	4	198.1	0.164	−30.88	70.8	1.10
F6	350	80	4	227.1	0.169	−29.08	83.0	0.94
F7	250	80	10	204.3	0.165	−25.73	62.4	2.26
F8	350	80	10	221.2	0.156	−21.78	81.7	2.02
F9	300	60	4	203.3	0.193	−27.18	62.7	0.81
F10	300	100	4	187.0	0.168	−38.28	77.4	0.97
F11	300	60	10	220.7	0.154	−29.61	75.0	2.39
F12	300	100	10	177.5	0.154	−24.65	69.5	2.24
F13	300	80	7	227.2	0.155	−32.09	71.2	1.63
F14	300	80	7	219.7	0.194	−29.93	80.6	1.87
F15	300	80	7	219.7	0.147	−28.52	74.7	1.61