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. 2018 Mar 1;8:3894. doi: 10.1038/s41598-018-22183-2

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© The Author(s) 2018, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of aggregated proteins in mRNA surveillance mutant strains. (A) Protein aggregates were isolated from the wild-type and mRNA surveillance mutant strains and analysed by SDS-PAGE and silver staining. (B) Proteins within insoluble aggregate fractions were identified by LC-MS and Venn diagrams show pairwise comparisons of the aggregated proteins in the wild-type and mutant strains. (C) Venn diagram comparing the aggregated proteins in the wild-type and mRNA surveillance mutant strains. 198 proteins were identified that aggregate in all five strains and are referred to as the Common-set.