Fig. 1.

Exposure of breast cancer cells to hyperglycaemia and fibronectin induces EMT. (a, i) MCF-7 cells were cultured as described in Materials and methods section. Western blotting was performed to examine the protein abundance of the indicated EMT markers. (a, ii) Densitometry was performed to quantify the protein levels of EMT markers. (b) SYBR green-based qPCR analysis of SLUG mRNA levels. (c) Changes in cell growth were assessed by direct count of viable cells in a haemocytometer (upper panel) and assessment of cyclin D1 protein expression by Western blotting (lower panel). (d) Cell invasion was measured using the transwell assay. In all cases, results shown are representative of three independent experiments, each performed in triplicate and are expressed as means of ±SEM. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)