Fig. 5.

Silencing of caveolin-1 prevents the increase in cell invasion and SLUG mRNA levels triggered by fulvestrant during hyperglycaemia-induced, matrix-specific EMT. (a, i&ii) Western blot analysis of caveolin-1 protein expression after siRNA-mediated silencing of FASN or ERα and following treatment with antiestrogens fulvestrant and tamoxifen. (b) The invasive capacity induced by fulvestrant was assessed in MCF-7 cells with or without caveolin-1 knocked down with siRNA using transwell invasion assay. (c) The effect of caveolin-1 knock-down on the fulvestrant-induced SLUG mRNA levels as determined by qPCR. (d, i&ii) Caveolin-1 knock-down efficiency was determined by western blotting. Results shown are representative of three independent experiments, each performed in triplicate. Data are represented as mean ± SEM.