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. 2018 Mar 1;9:895. doi: 10.1038/s41467-018-03289-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overexpression of hTET1cd-induced global CG demethylation in A. thaliana. a Bar graph of global methylation levels in two Col-0 WT replicates, two 35S:TET1 transgenic individuals, and met1. b Metaplot of CG methylation levels (100 kb windows) across five A. thaliana chromosomes. Methylation level differences were defined relative to Col-0 WT-1, and Col-0 WT-2 was used to assess background interference. Genome browser view of methylome profile of two regions (c, d) of the A. thaliana genome (purple vertical lines = CG methylation, blue vertical lines = CHG methylation, and gold vertical lines = CHH methylation). Metagene plots of CG methylation level across e gene bodies and f transposable elements. g Heat map of CG methylation level of CG DMRs. Bar plots of CHG (h) and CHH (i) methylation levels of CG DMRs that possess non-CG methylation in wild-type individuals