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. 2018 Mar 1;8:3846. doi: 10.1038/s41598-018-22252-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design and characterization of Axl synNotch in human Jurkat T cells. (A) Axl synNotch design. TF, transcriptional factor (tTA); TFBD, transcriptional factor binding domain. (B) The expression level of the Axl synNotch in Jurkat T cells. The myc-tag was stained with an anti-myc antibody for the measurement of surface synNotch expression. Control indicates non-transfected Jurkat cells that containing only tTA responsive reporter. (C) Axl synNotch response from plate bound Axl protein activation. Control indicates Jurkat T cells harboring synNotch responsible BFP reporter without Axl synNotch. (D) Axl synNotch activation via co-culturing of Axl+/Axl− K562 target cells. (E) BFP fluorescence level after co-culturing of Axl synNotch expressing Jurkat T cells with target K562 cells for 24 hr. Control indicates Jurkat T cells harboring only BFP reporter without Axl synNotch receptor. (F) IL-10 production level when co-cultured Jurkat T cells harboring tTA responsive IL-10 reporter cells with K562 cells for 24 hr. (None, no target cell or no effector cell condition. N.D., not detected: Data are representative of three biological replicates and presented as the mean ± SD).