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. 2018 Mar 1;8:3885. doi: 10.1038/s41598-018-22056-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitatively analyses of α-tubulin cytoskeleton arrangement and PI uptake induced by sonoporation with different microbubble-cell parameters R_D/d and constant p− = 0.4 MPa: (a) The values of asymptotic fluorescence intensity; (b) the values of characteristic decay time and recovery time; (c) a correlation plot showing the relationship between increment of PI fluorescence intensity and decay of GFP fluorescence intensity; and (d) a correlation plot showing the relationship between τ_PI and τ_GFP . Linear correlation coefficients are also indicated.