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. 2018 Mar 1;8:3855. doi: 10.1038/s41598-018-21729-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature and locomotor effects of H₂S compared to hypoxia. (A) Thermal images of H₂S-exposed mice in 21% O₂ (top row), in 17% O₂ (middle row), and hypoxia-subjected mice (bottom row) before (baseline), during, and after (recovery) exposure. The color of the animals reflects their temperature (scale bar). (B) The difference between the maximum superficial temperature (Δ max. superficial T) of the hypoxia (F_iO₂ 5% and 17%) and H₂S (F_iO₂ 17% and 21%) groups versus the normoxia group (F_iO₂ 21%) was plotted as a function of time before exposure (up to 0 h), during exposure (0–6 h), and after exposure (6–9 h). (C) Mouse mean locomotor activity per time point per group plotted as a function of time before exposure (up to 0 h), during exposure (0–6 h), and after exposure (6–9 h). Locomotor activity was derived from temporal changes in pixel grayscale intensity as described in the online supplemental information. In (B) and (C) the means ± SEM are plotted for N = 12/group (group A, C and E) or N = 6/group (group B and D) up to 6 h, and for N = 6/group from 6 to 9 h. Statistically significant intergroup differences are displayed under the corresponding plots.