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. 2018 Mar 1;8:3897. doi: 10.1038/s41598-018-22304-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Two steps in image analysis process to estimate individual mass from images as volume of revolution. (a) Bolbosoma capitatum picture with Feret diameter aligned with image wide margin (i.e. angle minimised); (b) binary conversion of the specimen after thresholding, white pixels represent the background and black pixels the animal surface. Scale bar 5 cm.