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. 2018 Mar 1;9(3):347. doi: 10.1038/s41419-018-0376-5

Fig. 2. Effects of WBP2 on cell growth and cell cycle progress of U251.

Fig. 2

a–d After seeding for 24 h, U251 and U87 cells (eight groups of cells) were processed with MTT assay (EGFP-Vector, EGFP-WBP2, siNC, and siWBP2) at 0, 24, 48, and 72 h. e Cell cycle analysis of U251 cells transfected with EGFP-WBP2, EGFP-Vector, siNC and siWBP2. f Cell cycle analysis of U87 cells transfected with EGFP-WBP2, EGFP-Vector, siNC and siWBP2. g, h Representative quantity of cell numbers in S-phase in the eight group cells. EGFP-Vector, control stable cell line transfected with pEGFP-C1 plasmid in U251 or U87 cells; pEGFP-WBP2, stable cell lines transfected with pEGFP-WBP2; siNC, cells transfected with negative control siRNA; siWBP2, downregulation of WBP2 expression transfected with siRNA of WBP2 in U251 or U87 cells. *P < 0.05, **P < 0.01