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. 2018 Mar 1;8:3891. doi: 10.1038/s41598-018-22113-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bufalin inhibits β-catenin/TCF signaling in HCC cells. (A) Bufalin induced the redistribution of β-catenin from nuclei to cytoplasm. Red immunofluorescence staining of β-catenin was shown in representative images and nuclei were counterstained with DAPI. (B) Bufalin inactivated β-catenin signaling in a dose-dependent manner. Western blot analysis was performed to detect the expression of active and total β-catenin, CCND1, EGFR and PCNA. β-actin was used as a loading control. (C) Bufalin decreased CCND1 and EGFR mRNA levels, but not CTNNB1, in a dose-dependent manner. Various concentrations of bufalin were incubated with PLC5 cells and quantitative RT-PCR was performed to detect transcript levels. ***P < 0.001.