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Thermodynamic parameters for the interaction between AcrIIA4 and SpyCas9 obtained by isothermal titration calorimetry at 25 °C.
| Description | KD (nM) | ΔG (kcal/mol) | ΔH (kcal/mol) | −TΔS (kcal/mol) |
|---|---|---|---|---|
| AcrIIA4:SpyCas9 | 4800 ± 150 | −7.3 ± 0.1 | −11.5 ± 0.7 | 4.2 ± 0.8 |
| AcrIIA4:SpyCas9‒sgRNA | 0.6 ± 0.1 | −12.6 ± 0.4 | −10.3 ± 0.1 | −2.3 ± 0.4 |
