Fig. 5.

Sulfatide promotes cellular uptake of MANF. a Exemplar western blot of lysates from target HEK293T cells treated with Hs-MANF or Hs-MANF (K112L) (purified from conditioned PBS of HEK293T-MANF::V5 cell lines) with or without sulfatide. b Exemplar western blot of lysates from target HEK293T cells treated with Hs-MANF (purified from conditioned PBS of HEK293T-MANF::V5 cell lines) with increasing doses of sulfatide added to the target HEK293T cell medium. c Exemplar immunofluorescence images of endogenous GPR78 (green) and V5-stained Hs-MANF (red), merged image with DAPI after incubation with Hs-MANF (purified from conditioned PBS of HEK293T-MANF::V5 cell lines) and with V5 staining of no-sulfatide control. Arrow heads indicate representative loci of co-localization. Scale bar, 20 µm