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. 2018 Mar 1;8:3887. doi: 10.1038/s41598-018-21502-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal microscope images of SKBR3 and SVR cells incubated with dual-targeted NB_D. (a–c) SKBR3 cells were substantively stained by nanoparticle-antibody bioconjugates (green, red or yellow, incomplete circles) around cell nuclei. (d–f) SVR cells were strongly stained by nanoparticle-antibody bioconjugates (bright, complete green, red or yellow circles). Green, fluorescence signal of anti-HER2 antibody labeled with FITC. Red, fluorescence signal of anti-VEGFR2 antibody labeled with PE. Yellow, merged fluorescence of red and green. Blue, nuclei stained with DAPI.