TABLE 1.
Primers used for cloning of HILPDA variants, HSL and ATGL
| Construct | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Vector |
| hHILPDA_1-63 | GTATTTTCAGGGCGCCATGGCCATGAAGCATGTGTTGAACC | AGTGGTGGTGGTGGTGGTGCCAACGGTGCTCAGCTTGTC | pSumo |
| hHILPDA_1-20 | CTCCATCTTCTAGAGAGTGATGTAGTCCC | AGTAGGGTCAGTACCACAC | pSumo |
| hHILPDA_1-24 | TAGAGTGATGTGATCCCTAGAGGGCTTAC | ACGAAGATGGAGAGTAGG | pSumo |
| hHILPDA_1-28 | GTCCCTAGAGTGATTACTAGAGAG | TCCATCACTCTAACGAAG | pSumo |
| hHILPDA_20-63 | GTATTTTCAGGGCGCCATGGGCTTCGTTAGAGTGATGG | AGTGGTGGTGGTGGTGGTGCTCACATGCTTCTGGATGG | pSumo |
| hHILPDA_25-63 | GAGTCCCTAGAGGGCTTACTAGAG | GCCCATGGCGCCCTGAAA | pSumo |
| hHILPDA 1-63 | GCATGGACGAGCTGTACAAGGAAAACCTGTATTTTCAGGG | GGCTGATTATGATCAGTTATTCAGTGATGGTGATGGTGATGCATGCTTCTGGATGGATG | pGFPC1 |
| mHSL | GTCACTCGAGGCCACCATGGATTTACGCACGATGAC | GACTCCGCGGGTTCAGTGGTGCAGCAGGCG | pECFP-N1 |
| mATGL_1-288 | AGGTGGCGGAGAAAACCTGTATTTTCAGGGCATG | GCGGGTGGCTCCAAGCGCTGCCGGCATCTTCTTCGCCGGCACG | pST50 |
| mHILPDA | GCCTCGAGACCATGAAGTTCATGCTGAAC | ATGGTACCCTGCACTCCTCGGGATGG | pEGFP-N2 |
| mATGL | GCCTCGAGACCATGTTCCCGAGGGAGACCAAG | ATGGTACCGCAAGGCGGGAGGCCAGG | pEGFP-N2 |
| mCherry | ATGGTACCATGGTGAGCAAGGGCGAG | AGCGGCCGCTCACTTGTACAGCTCGTC | pEGFP-N2 |
| sYFP2 | ATGGTACCATGGTGAGCAAGGGCGAG | AGCGGCCGCTTACTTGTACAGCTCGTC | pEGFP-N2 |
| mTurquoise2 | ATGGTACCATGGTGAGCAAGGGCGAG | AGCGGCCGCTCACTTGTACAGCTCGTC | pEGFP-N2 |