Fig. 6.
PC transfer activity of StarD7 is negatively influenced by ceramide. A: Time course of PC transfer by StarD7 and StarD7R189Q (top) and of ceramide transfer by CERT START and CERT STARTN504A (bottom). Lipid transfer from donor liposomes containing 10 mol% PC or ceramide was measured as described in the Materials and Methods. Data are shown as the mean ± error range of two or three independent experiments, as indicated. B: Quantitative analysis of the PC (top) and ceramide (bottom) transfer activity of CERT, StarD7, and StarD7R189Q was performed as described in the Materials and Methods. Data are shown as the mean ± error range of two independent experiments. C: Quantitative analysis of the PC transfer activity of StarD2, StarD7, and StarD7R189Q using donor liposomes containing 5 mol% PC in the absence (−) or presense of 10 mol% ceramide (Cer) or DAG. The analysis was performed as described in the Materials and Methods. Data are shown as the mean ± SD of at least three independent experiments. ***P < 0.001; paired t-test.