Fig. 2.

Activation of LXR regulates CH25H protein stability and induces CH25H mRNA expression. A: HepG2 cells were treated with cycloheximide (CHX, 5 μM), CHX (5 μM) plus T317 (100 nM), or 25-HC (2 μM) for the indicated times. Total cellular proteins were extracted and CH25H protein expression was determined by Western blot. ^aP < 0.05 vs. control; ns: not significantly different (n = 3). B, C: HepG2 cells were treated with T317 or 25-HC at the indicated concentrations for 16 h (B) or with T317 (100 nM) or 25-HC (4 μM) for the indicated times (C). Total cellular RNA was extracted and CH25H mRNA expression was determined by quantitative real-time-PCR. *P < 0.05 vs. control in the corresponding group (n = 3).