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. 2018 Jan 3;59(3):439–451. doi: 10.1194/jlr.M080440

Fig. 5.

Fig. 5.

T317 and 25-HC regulate expression of the genes for biosynthesis of cholesterol or triglyceride and cholesterol efflux in HepG2 cells. A, B, E: HepG2 cells were treated with T317 and 25-HC for 16 h. Expression of cholesterol biosynthesis related genes [precursor of SREBP2 (P) and mature SREBP2 (M), HMGCR] (A), triglyceride biosynthesis related genes (SREBP1 and FASN) (B), and cholesterol efflux related genes (ABCA1 and ABCG1) (E) were determined by Western blot. aP < 0.05, bP < 0.01 vs. control (n = 3). C, D: HepG2 cells were treated with T317 (200 nM) or 25-HC (2 μM) for 16 h followed by determination of cellular total cholesterol (CHO) levels (C) and triglyceride (TG) levels (D) using assay kits. *P < 0.05; **P < 0.01 vs. control in the corresponding groups (n = 3).