Fig. 5.

Immunoblot analysis of livers from sucrose-refed control and L-Chrebp^−/− mice treated with AAV-GFP or AAV-nSREBP-1c. Littermate control and L-Chrebp^−/− mice (same as those described in supplemental Table S3) were injected via the tail vein with recombinant AAV-CAG-GFP (GFP) or AAV-CAG-nSREBP-1c (nBP-1c) (2 × 10¹¹ gene copies per mouse). Seven days after the injection, the mice were fasted for 12 h (F) or fasted for 12 h and then refed (R) with high-sucrose diet for 12 h prior to study. Liver whole-cell lysates were prepared individually, and equal amounts of protein from each mouse of the same group (four to six per group) were pooled. Aliquots (40 μg) of the pooled protein were subjected to SDS-PAGE and immunoblot analysis.