A low dietary ω6:ω3 ratio enhances body weight loss in response to a β3-adrenergic receptor agonist. Mice maintained at 28°C were fed with diets supplemented with ω6 PUFAs or ω3 PUFAs for 12 weeks. CL316,243 or vehicle (NaCl) treatment was performed daily during the last week of feeding. A: Body weight development of mice (left); body weight loss of mice after CL316,243 treatment relative to vehicle treatment (right). B: Interscapular brown adipose tissue (iBAT) and epidydimal white adipose tissue (eWAT) weight loss after CL316,243 treatment. C: Plasma glycerol levels after NaCl or CL316,243 treatment. Data are mean ± SEM, n = 12 mice/group. a, P < 0.01 ω6 versus ω3; b, P < 0.01 NaCl versus CL316,243.