Figure 1.

Genes related to histone methylation modification were up‐regulated in hypoxia‐induced LX‐2 cells. Total RNA was extracted and reversely transcribed into cDNA from normal oxygen or CoCl₂‐treated hypoxia‐induced human hepatic stellate cell line LX‐2. (A) Cluster analysis of genomewide expression chips (red: up‐regulated genes, green: down‐regulated genes). (B) The expression of Ogt, a representative up‐regulated gene from genomewide expression chips, was detected at mRNA level by qPCR. Densitometric analysis was performed using pooled data from three such experiments. Data were mean ± SD (*P < 0.05). (C) Total protein was extracted at 0, 1, and 8 h from hypoxia‐induced LX‐2 cells, and the expression of OGT was detected by western blot. Densitometric analysis was performed using pooled data from three such experiments. Data were mean ± SD (*P < 0.05). (D) BALB/c female mice, 6–8 weeks old, were percutaneously infected with 25 cercariae of Schistosoma japonicum through the shaved abdomen, sacrificed at 8 weeks postinfection, and samples of liver were collected. The expression of OGT in S. japonicum‐infected (n = 3) and noninfected (n = 3) mice liver was detected with immunohistochemistry and representative images were shown.