Figure 2.
H3K4me3 histone methylation modification occurred in Hif‐1 transcriptional complex in hypoxia‐induced LX‐2 cells. (A) LX‐2 cells were treated with 100 μm CoCl2. The cytoplasmic and nuclear protein was extracted at indicated time. Hif‐1α and H3K4me3 were detected by western blot. (B) Densitometric analysis of Hif‐1α and H3K4me3 expression was performed using pooled data from three such experiments. Results were expressed as mean ± SD, *P < 0.05, **P < 0.01. (C) LX‐2 cells were treated with 100 μm CoCl2, and 1 mg of cell lysates was collected at indicated time. Anti‐Hif‐1α or anti‐H3K4me3 monoclonal antibody was, respectively, added to cell lysates, and immune‐precipitated proteins were then detected with anti‐H3K4me3 or anti‐Hif‐1α antibody by western blot.