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. 2018 Feb 7;4(2):230–234. doi: 10.1021/acscentsci.7b00554

Figure 3.

Figure 3

Inhibition of strigolactone signaling by DL1. (a) qRT-PCR analysis for the expression level of strigolactone-responsive genes, BRC1 and STH7. The 10-day-old wild-type Arabidopsis seedlings were treated with 1 μM (+)-GR24 and 20 μM DL1 for 24 h. The relative values are normalized to ACT2. The value means ± SE (n = 3 biological replicates). (b, c) Bright field (top) and fluorescence (bottom) images of the hypocotyl (b) and root (c) in 2-week-old 35S::AtD14-GFP/atd14–1 seedlings treated with 1 μM (+)-GR24 and indicated concentration of DL1 for 24 h. Numerals at the upper right indicate the number of plants showing the figure phenotype per total number of plants examined.